{"created":"2024-03-05T06:14:44.693137+00:00","id":2012910,"links":{},"metadata":{"_buckets":{"deposit":"dcc601d1-b8ac-46a1-a53e-2e71ed266035"},"_deposit":{"created_by":9,"id":"2012910","owners":[9],"pid":{"revision_id":0,"type":"depid","value":"2012910"},"status":"published"},"_oai":{"id":"oai:our.repo.nii.ac.jp:02012910","sets":["1623632832836:1707978071892","1707979148924:1707983962284"]},"author_link":[],"item_30002_access_rights4":{"attribute_name":"Access Rights","attribute_value_mlt":[{"subitem_access_right":"metadata only access","subitem_access_right_uri":"http://purl.org/coar/access_right/c_14cb"}]},"item_30002_creator2":{"attribute_name":"Creator","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"吉村, 彰","creatorNameLang":"ja"},{"creatorName":"ヨシムラ, アキラ","creatorNameLang":"ja-Kana"},{"creatorName":"Yoshimura, Akira","creatorNameLang":"en"}]}]},"item_30002_date11":{"attribute_name":"Date","attribute_value_mlt":[{"subitem_date_issued_datetime":"2014-01-01","subitem_date_issued_type":"Issued"}]},"item_30002_date_granted32":{"attribute_name":"Date Granted","attribute_value_mlt":[{"subitem_dategranted":"2014-06-17"}]},"item_30002_degree_grantor33":{"attribute_name":"Degree Grantor","attribute_value_mlt":[{"subitem_degreegrantor":[{"subitem_degreegrantor_language":"ja","subitem_degreegrantor_name":"大分大学"}]}]},"item_30002_degree_name31":{"attribute_name":"Degree Name","attribute_value_mlt":[{"subitem_degreename":"博士(医学)","subitem_degreename_language":"ja"}]},"item_30002_description9":{"attribute_name":"Description","attribute_value_mlt":[{"subitem_description":"In mammalian hearts, Purkinje cells varied greatly in morphological appearance in different species, and were divided into three groups. Bovine Purkinje cells corresponding to group I were a large size, and had a few myofibrils and abundant intermediate filaments throughout the cytoplasm. The aim of the present study was to clarify the more detailed distribution and three-dimensional architecture of intermediate filaments in Purkinje cells. The hearts in various mammals including humans were investigated by both immuno-histochemistry and scanning electron microscopy (SEM).Immuno-histochemical studies demonstrated that sheep Purkinje cells in group I had a great number of intermediate filaments of 10 nm positive for desmin antibody. Purkinje cells in group II (humans, monkeys and dogs) and group III (mice) were somewhat larger or smaller in size than myocardial cells, but also showed a strong positive reaction for desmin antibody. The saponin or NaOH treatment of cardiac tissues in sheep and humans enabled us to view intermediate filaments by SEM three-dimensionally. Intermediate filaments in sheep Purkinje cells formed a considerably delicate network, and were distributed throughout the cytoplasm. In contrast, those in human Purkinje cells were lower in density, and were present around the nucleus and between myofibrils. It was concluded that a delicate network of intermediate filaments in Purkinje cells of mammalian hearts acted as the cytoskeleton to maintain intercellular stability.","subitem_description_language":"en"}]},"item_30002_dissertation_number30":{"attribute_name":"Dissertation Number","attribute_value_mlt":[{"subitem_dissertationnumber":"乙第331号"}]},"item_30002_identifier16":{"attribute_name":"Identifier","attribute_value_mlt":[{"subitem_identifier_type":"HDL","subitem_identifier_uri":"http://hdl.handle.net/10559/17164"}]},"item_30002_language12":{"attribute_name":"Language","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_30002_publisher10":{"attribute_name":"Publisher","attribute_value_mlt":[{"subitem_publisher":"大分大学","subitem_publisher_language":"ja"}]},"item_30002_relation18":{"attribute_name":"Relation","attribute_value_mlt":[{"subitem_relation_type":"isVersionOf","subitem_relation_type_id":{"subitem_relation_type_id_text":"https://doi.org/10.1007/s00795-014-0069-9","subitem_relation_type_select":"DOI"}}]},"item_30002_resource_type13":{"attribute_name":"Resource Type","attribute_value_mlt":[{"resourcetype":"doctoral thesis","resourceuri":"http://purl.org/coar/resource_type/c_db06"}]},"item_30002_title0":{"attribute_name":"Title","attribute_value_mlt":[{"subitem_title":"Immuno-histochemistry and three-dimensional architecture of the intermediate filaments in Purkinje cells in mammalian hearts","subitem_title_language":"en"},{"subitem_title":"哺乳動物心臓のプルキンエ細胞における中間径フィラメントの免疫組織化学的検索と三次元構築","subitem_title_language":"ja"}]},"item_title":"Immuno-histochemistry and three-dimensional architecture of the intermediate filaments in Purkinje cells in mammalian hearts","item_type_id":"30002","owner":"9","path":["1707983962284","1707978071892"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2023-04-09"},"publish_date":"2023-04-09","publish_status":"0","recid":"2012910","relation_version_is_last":true,"title":["Immuno-histochemistry and three-dimensional architecture of the intermediate filaments in Purkinje cells in mammalian hearts"],"weko_creator_id":"9","weko_shared_id":-1},"updated":"2024-03-18T05:51:16.312832+00:00"}