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アイテム
子宮内膜症においてエピジェネティクスにより発現が抑制されているmiR-503は,子宮内膜症間質細胞に対してアポトーシス促進、細胞周期停止,細胞増殖抑制,血管新生抑制,収縮能抑制に働く
http://hdl.handle.net/10559/17170
http://hdl.handle.net/10559/1717007c94f3b-2ee1-4c65-82f4-48e3b9998c5c
| Item type | デフォルトアイテムタイプ（フル）(1) | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| 公開日 | 2023-04-07 | |||||||||||
| タイトル | ||||||||||||
| タイトル | miR-503, a microRNA Epigenetically Repressed in Endometriosis, Induces Apoptosis and Cell-cycle Arrest and Inhibits Cell Proliferation, Angiogenesis, and Contractility of Human Ovarian Endometriotic Stromal Cells | |||||||||||
| 言語 | en | |||||||||||
| タイトル | ||||||||||||
| タイトル | 子宮内膜症においてエピジェネティクスにより発現が抑制されているmiR-503は,子宮内膜症間質細胞に対してアポトーシス促進、細胞周期停止,細胞増殖抑制,血管新生抑制,収縮能抑制に働く | |||||||||||
| 言語 | ja | |||||||||||
| 作成者 |
平川, 東望子
× 平川, 東望子
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| アクセス権 | ||||||||||||
| アクセス権 | metadata only access | |||||||||||
| アクセス権URI | http://purl.org/coar/access_right/c_14cb | |||||||||||
| 内容記述 | ||||||||||||
| 内容記述 | Study question: Is the micro-RNA (miRNA) miR-503, downregulated in endometriotic cyst stromal cells (ECSCs) and does this affect the cell cycle, cell proliferation, angiogenesis and contractility of these cells? SUMMARY ANSWER: miR-503 expression is downregulated in ECSCs by DNA hypermethylation and this contributes to their proliferation, resistance to apoptosis, extracellular matrix (ECM) contractility and angiogenesis through effects on cyclin D1, B-cell lymphoma/leukemia (Bcl)-2, Ras homology A and vascular endothelial growth factor A (VEGF-A). | |||||||||||
| 言語 | en | |||||||||||
| 内容記述 | ||||||||||||
| 内容記述 | What is known already: A variety of miRNAs are demonstrated to involve in the pathogenesis of endometriosis. miR-503 is a miRNA with tumor-suppressor functions, whose expression is suppressed in ECSCs. | |||||||||||
| 言語 | en | |||||||||||
| 内容記述 | ||||||||||||
| 内容記述 | Study design, size, duration: We isolated ECSCs and normal endometrial stromal cells (NESCs) from ovarian endometriotic tissues (n = 32) and eutopic endometrial tissues without endometriosis (n = 8), respectively. | |||||||||||
| 言語 | en | |||||||||||
| 内容記述 | ||||||||||||
| 内容記述 | Participants/materials, setting, methods: We investigated the functions of miR-503 by using miR-503-transfected ECSCs and the DNA methylation status of miR-503 gene in ECSCs and NESCs by combined bisulfite restriction analysis. | |||||||||||
| 言語 | en | |||||||||||
| 内容記述 | ||||||||||||
| 内容記述 | Main results and the role of chance: In ECSCs, miR-503 is downregulated by the DNA hypermethylation of its gene. The transfection of miR-503 into ECSCs resulted in the inhibition of cell proliferation and induction of cell-cycle arrest at G0/G1 phase through the suppression of cyclin D1, the induction of apoptosis through Bcl-2 suppression, the inhibition of VEGF-A production and the attenuation of ECM contractility via the suppression of Rho/Rho-associated coiled-coil-forming protein kinase-pathways. | |||||||||||
| 言語 | en | |||||||||||
| 内容記述 | ||||||||||||
| 内容記述 | Large scale data: NA. | |||||||||||
| 言語 | en | |||||||||||
| 内容記述 | ||||||||||||
| 内容記述 | Limitations, reasons for caution: The present experiments were carried out only with the stromal component of endometriosis and eutopic endometrium. The experiments with the eutopic endometrial stromal cells from women with endometriosis are not performed. | |||||||||||
| 言語 | en | |||||||||||
| 内容記述 | ||||||||||||
| 内容記述 | Wider implications of the findings: Our findings indicate that epigenetically repressed miR-503 in ECSCs is involved in the acquisition of endometriosis-specific cellular functions. | |||||||||||
| 言語 | en | |||||||||||
| 内容記述 | ||||||||||||
| 内容記述 | Study funding/competing interests: This work was supported in part by Grants-in-Aid for Scientific Research from the Japan Society for the Promotion of Science (no. 13237327 to K.N., no. 26861335 to K.K. and no. 23592407 to H.N.) and the Kanzawa Medical Research Foundation (to K.K.). There are no conflicts of interest to declare. | |||||||||||
| 言語 | en | |||||||||||
| 出版者 | ||||||||||||
| 出版者 | 大分大学 | |||||||||||
| 言語 | ja | |||||||||||
| 日付 | ||||||||||||
| 日付 | 2017-01-01 | |||||||||||
| 日付タイプ | Issued | |||||||||||
| 言語 | ||||||||||||
| 言語 | eng | |||||||||||
| 資源タイプ | ||||||||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_db06 | |||||||||||
| 資源タイプ | doctoral thesis | |||||||||||
| 識別子 | ||||||||||||
| 識別子 | http://hdl.handle.net/10559/17170 | |||||||||||
| 識別子タイプ | HDL | |||||||||||
| 関連情報 | ||||||||||||
| 関連タイプ | isVersionOf | |||||||||||
| 識別子タイプ | DOI | |||||||||||
| 関連識別子 | https://doi.org/10.1093/humrep/dew217 | |||||||||||
| 学位授与番号 | ||||||||||||
| 学位授与番号 | 甲第580号 | |||||||||||
| 学位名 | ||||||||||||
| 言語 | ja | |||||||||||
| 学位名 | 博士(医学) | |||||||||||
| 学位授与年月日 | ||||||||||||
| 学位授与年月日 | 2017-03-23 | |||||||||||
| 学位授与機関 | ||||||||||||
| 言語 | ja | |||||||||||
| 学位授与機関名 | 大分大学 | |||||||||||
