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哺乳動物心臓のプルキンエ細胞における中間径フィラメントの免疫組織化学的検索と三次元構築
http://hdl.handle.net/10559/17164
http://hdl.handle.net/10559/171640adfab9d-0b00-4fbf-a008-7e0513e7b256
| Item type | デフォルトアイテムタイプ（フル）(1) | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| 公開日 | 2023-04-09 | |||||||||||
| タイトル | ||||||||||||
| タイトル | Immuno-histochemistry and three-dimensional architecture of the intermediate filaments in Purkinje cells in mammalian hearts | |||||||||||
| 言語 | en | |||||||||||
| タイトル | ||||||||||||
| タイトル | 哺乳動物心臓のプルキンエ細胞における中間径フィラメントの免疫組織化学的検索と三次元構築 | |||||||||||
| 言語 | ja | |||||||||||
| 作成者 |
吉村, 彰
× 吉村, 彰
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| アクセス権 | ||||||||||||
| アクセス権 | metadata only access | |||||||||||
| アクセス権URI | http://purl.org/coar/access_right/c_14cb | |||||||||||
| 内容記述 | ||||||||||||
| 内容記述 | In mammalian hearts, Purkinje cells varied greatly in morphological appearance in different species, and were divided into three groups. Bovine Purkinje cells corresponding to group I were a large size, and had a few myofibrils and abundant intermediate filaments throughout the cytoplasm. The aim of the present study was to clarify the more detailed distribution and three-dimensional architecture of intermediate filaments in Purkinje cells. The hearts in various mammals including humans were investigated by both immuno-histochemistry and scanning electron microscopy (SEM).Immuno-histochemical studies demonstrated that sheep Purkinje cells in group I had a great number of intermediate filaments of 10 nm positive for desmin antibody. Purkinje cells in group II (humans, monkeys and dogs) and group III (mice) were somewhat larger or smaller in size than myocardial cells, but also showed a strong positive reaction for desmin antibody. The saponin or NaOH treatment of cardiac tissues in sheep and humans enabled us to view intermediate filaments by SEM three-dimensionally. Intermediate filaments in sheep Purkinje cells formed a considerably delicate network, and were distributed throughout the cytoplasm. In contrast, those in human Purkinje cells were lower in density, and were present around the nucleus and between myofibrils. It was concluded that a delicate network of intermediate filaments in Purkinje cells of mammalian hearts acted as the cytoskeleton to maintain intercellular stability. | |||||||||||
| 言語 | en | |||||||||||
| 出版者 | ||||||||||||
| 出版者 | 大分大学 | |||||||||||
| 言語 | ja | |||||||||||
| 日付 | ||||||||||||
| 日付 | 2014-01-01 | |||||||||||
| 日付タイプ | Issued | |||||||||||
| 言語 | ||||||||||||
| 言語 | eng | |||||||||||
| 資源タイプ | ||||||||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_db06 | |||||||||||
| 資源タイプ | doctoral thesis | |||||||||||
| 識別子 | ||||||||||||
| 識別子 | http://hdl.handle.net/10559/17164 | |||||||||||
| 識別子タイプ | HDL | |||||||||||
| 関連情報 | ||||||||||||
| 関連タイプ | isVersionOf | |||||||||||
| 識別子タイプ | DOI | |||||||||||
| 関連識別子 | https://doi.org/10.1007/s00795-014-0069-9 | |||||||||||
| 学位授与番号 | ||||||||||||
| 学位授与番号 | 乙第331号 | |||||||||||
| 学位名 | ||||||||||||
| 言語 | ja | |||||||||||
| 学位名 | 博士(医学) | |||||||||||
| 学位授与年月日 | ||||||||||||
| 学位授与年月日 | 2014-06-17 | |||||||||||
| 学位授与機関 | ||||||||||||
| 言語 | ja | |||||||||||
| 学位授与機関名 | 大分大学 | |||||||||||
